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TABLE OF CONTENTS
cell membrane
Lipid docking assay: fluorescence . . . . . . . . . . . . . . . . . . . . . . 9
Close is not enough: SNARE-dependent membrane fusion requires an
active mechanism that transduces force to membrane anchors . . . . . . 9
Cell adherence: absorbance . . . . . . . . . . . . . . . . . . . . . . . . . 9
2´-Hydroxychalcone inhibits nuclear factor-B and blocks tumor
necrosis factor -- and lipopolysaccharide-induced adhesion of
neutrophils to human umbilical vein endothelial cells . . . . . . . . . . 9
cell signaling
G-protein coupled receptors: luminescence . . . . . . . . . . . . . . . . 10
Ca
++
-induced G protein-coupled receptor (GPCR) activation
monitored via aequorin luminescence in the LMax
TM
microplate
luminometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
biochemistry
enzymes: absorbance
Adenylate kinase/NADH . . . . . . . . . . . . . . . . . . . . . . . . . 13
The pro-apoptotic proteins, Bid and Bax, cause a limited
permeabilization of the mitochondrial outer membrane that is
enhanced by cytosol . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Alcohol dehydrogenase, glutathione S-transferase & esterase activity . . . 13
Quantitative kinetic assays for glutathione S-transferase and general
esterase in individual mosquitoes using an EIA reader . . . . . . . . . 13
!
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-galactosidase . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Action of the thiamine antagonist bacimethrin on thiamine biosynthesis . 14
HveA (herpesvirus entry mediator A), a coreceptor for
herpes simplex
virus entry, also participates in virus-induced cell fusion . . . . . . . . 14