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This document is a cache from http://labs.idi.harvard.edu/springer/uploads/Protocols/Equipment%20Manuals/ELISAplatereadermaual.pdf


applications guide to microplate systems

Document source : labs.idi.harvard.edu


Spectrosoft System
User Guide

MTX Lab Systems Inc 3-23

Column Mean Blank

To calculate well values against a mean blank of values read from a
designated column of blanks:
[1] Click the
check box and drag the
cup to the desired
blank column heading (1-12).


[2] Spectrosoft automatically averages the read values from the
designated column and then sets them to the calculated mean for final
calculating of the remaining wells read. Clicking on "Calibrate" displays
the results of the subtracted mean value from the raw values.

Row Mean Blank

To calculate the well values against a mean blank of values read from a
designated row of blanks:
[1] Click the
box and drag the blank cup to the desired
blank row.

[2] Spectrosoft automatically averages the blank values read from the
designated row and then sets them to the calculated mean for final
calculating of the remaining wells read. Clicking on "Calibrate" displays
the results of the subtracted mean value from the raw values.

Blocking

Blocking is a useful option to block out unneeded wells from view.

To Block out a single well
Drag and drop the Block cup
over the desired well to be blocked out,
The foreground color turns gray.

To remove a Block from a single well
Drag the Block cup
over the well, and the foreground color turns
blue.













Summary :

Spectrosoft System User Guide MTX Lab Systems Inc 3-23 Column Mean Blank To calculate well values against a mean blank of values read from a designated column of blanks: [1] Click the check box and drag the cup to the desired blank column heading (1-12).


Tags : alues,mean,blank,well,read,block,column,designated,drag,row,cup,spectrosoft,out





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