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Protein ID Solutions Sample Prep Guide
Contents
Protocol for spin cartridges
30
Troubleshooting
34
Tips
35
Cartridge specifications
36
4
Protein Concentration with Agilent Spin Concentrators
37
Available concentrators
38
Storage
39
Safety
39
Procedure summary
40
Additional materials required
41
Protocol for concentrating proteins and desalting/buffer exchange
42
Troubleshooting
43
Tips
43
Technical specifications
44
5
Guidelines for Protein Fractionation with 1D and 2D Gel Electrophoresis
45
To prepare samples for gel electrophoresis
47
To run the gels
47
To prepare gels for in-gel tryptic digestion
47
6
In-Gel Tryptic Digestion with Agilent Kit
49
Kit contents
50
Storage
50
Safety
51
Procedure summary
52
Additional materials required
54
Protocol for in-gel digestion
55
Solution preparation
59
Troubleshooting
63
Tips
63
References
65
Summary :
vi Protein ID Solutions Sample Prep Guide Contents Protocol for spin cartridges 30 Troubleshooting 34 Tips 35 Cartridge specifications 36 4 Protein Concentration with Agilent Spin Concentrators 37 Available concentrators 38 Storage 39 Safety 39 Procedure summary 40 Additional materials required 41 Protocol for concentrating proteins and desalting/buffer exchange 42 Troubleshooting 43 Tips 43 Technical specifications 44 5 Guidelines for Protein Fractionation with 1D and 2D Gel Electrophoresis 45 To prepare samples for gel electrophoresis 47 To run the gels 47 To prepare gels for in-gel tryptic digestion 47 6 In-Gel Tryptic Digestion with Agilent Kit 49 Kit contents 50 Storage 50 Safety 51 Procedure summary 52 Additional materials required 54 Protocol for in-gel digestion 55 Solution preparation 59 Troubleshooting 63 Tips 63 References 65
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protein,tips,troubleshooting,protocol,digestion,ingel,prepare,safety,summary,gel,agilent,spin,tryptic