Key Largo Sample Prep Guide.book

54 Protein ID Solutions Sample Prep Guide 6 In-Gel Tryptic Digestion with Agilent Kit Figure 8 Timeline for steps related to in-gel digestion Additional materials required In addition to the In-Gel Tryptic Digestion Kit, you need the following: · 600-µL microcentrifuge tubes (receiver tubes) · 50-mL capped bottle or equivalent · 10-mL storage bottle, tube or equivalent · Ultrapure water (18 megohm equivalent) · Micropipettors - 10, 100, 500, and 1000 µL · Pipette tips, thin gel loader type · Tube heater/shaker, such as Eppendorf Thermomixer Allow 3.5 continuous hours for these steps Destain 60 min Alkylate 60 min Wash 30 min Shrink Dry 25 min Reduce 10 min Start digestion immediately Swell 15 min Digest 4 hours or overnight