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Protein ID Solutions Sample Prep Guide
8
Peptide Cleanup with Agilent C18 Pipette Tips
Additional materials required
The following is a list of required materials.
· Ultrapure water (18 megohm equivalent)
· Acetonitrile (ACN), HPLC grade
· Trifluoroacetic acid (TFA), sequencing grade
· 10-µL adjustable micropipettors
· 10-µL standard pipette tips
· Sample vials or tubes
Solution preparation
Prepare the solutions listed in
Table 15
.
Table 15
Solution preparation
Solution
Purpose
Description
A
Sample preparation solution
0.5% TFA in water
B
Media wetting solution
ACN
C
Binding solution
0.1% TFA in water
D
Wash solution
0.1% TFA in water
E
Sample elution solution
0.1% TFA / 60% ACN
Summary :
Table 15 Solution preparation Solution Purpose Description A Sample preparation solution 0.5% TFA in water B Media wetting solution ACN C Binding solution 0.1% TFA in water D Wash solution 0.1% TFA in water E Sample elution solution 0.1% TFA / 60% ACN
Tags :
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